Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Specificity
Natural and recombinant Dog Pepsin A
Subcellular Location
Secreted
Detection Range
0.312-20ng/ml
Entryname
PEPA_CANFA
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pepsin A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.3%
Protein Name
Pepsin A
Recovery
Recovery was determined by spiking various levels of Pepsin A into serum and plasma .