ELISA Kit for Dog Heparin-binding growth factor 1

Mfr.No.

E0032d

Alternative Names

Acidic fibroblast growth factor ; aFGF ; Alpha-endothelial cell growth factor ; FGF-1 ; ECGF ; Heparin-binding growth factor 1 ; HBGF-1

Categories

Elisa

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

Specificity

Natural and recombinant Dog Heparin-binding growth factor 1

Subcellular Location

Secreted Cytoplasm Cytoplasm cell cortex Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.

Detection Range

78-5000pg/mL

Entryname

FGF1_CANFA

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-100%	97-104%	95-98%	91-96%
EDTA plasma(n=5)	89-93%	102-105%	94-97%	93-98%
heparin plasma(n=5)	91-95%	96-99%	106-108%	87-93%

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.8%

Protein Name

Heparin-binding growth factor 1

Recovery

Recovery was determined by spiking various levels of FGF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	93-97
Plasma	97	96-102

Sensitivity

32pg/mL

Sequence

15:

NYMKPKLLYX ; SNGGH

Sequence Length

Sub Unit

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13.

Uniprot	P18651
Gene Name	FGF1
Immunogen Species	Canis Lupus Familiaris (Dog)

Reference	1. "Isolation of heparin-binding growth factors from bovine, porcine and canine hearts." Quinkler W., Maasberg M., Bernotat-Danielowski S., Luethe N., Sharma H.S., Schaper W. Eur. J. Biochem.181:67-73(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE

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SKU	Supplier No.	Size	Your price	Quantity
BHE13804145	E0032d	96T	$900		Add to Cart

ELISA Kit for Dog Heparin-binding growth factor 1

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