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Home / Assay Kits / ELISA Kits

ELISA Kit for Chicken Vinculin

SKU : BHE13811844

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1839c
Alternative Names	Metavinculin ; VINC1
Categories	Elisa
Function	Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Specificity	Natural and recombinant Chicken Vinculin
Subcellular Location	Cytoplasm cytoskeleton Cell junction adherens junction Cell membrane Peripheral membrane protein Cytoplasmic side Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
Detection Range	3.12-200ng/mL
Entryname	VINC_CHICK
Method Type	Sandwich
Platform	PF01044
Protein Name	Vinculin
Sensitivity	0.67ng/mL
Sequence	50: MPVFHTRTIE ; SILEPVAQQI ; SHLVIMHEEG ; EVDGKAIPDL ; TAPVSAVQAA ; 100: VSNLVRVGKE ; TVQTTEDQIL ; KRDMPPAFIK ; VENACTKLVR ; AAQMLQADPY ; 150: SVPARDYLID ; GSRGILSGTS ; DLLLTFDEAE ; VRKIIRVCKG ; ILEYLTVAEV ; 200: VETMEDLVTY ; TKNLGPGMTK ; MAKMIDERQQ ; ELTHQEHRVM ; LVNSMNTVKE ; 250: LLPVLISAMK ; IFVTTKNTKS ; QGIEEALKNR ; NFTVEKMSAE ; INEIIRVLQL ; 300: TSWDEDAWAS ; KDTEAMKRAL ; ALIDSKMNQA ; KGWLRDPNAP ; PGDAGEQAIR ; 350: QILDEAGKAG ; ELCAGKERRE ; ILGTCKTLGQ ; MTDQLADLRA ; RGQGATPMAM ; 400: QKAQQVSQGL ; DLLTAKVENA ; ARKLEAMTNS ; KQAIAKKIDA ; AQNWLADPNG ; 450: GSEGEEHIRG ; IMSEARKVAE ; LCEEPKERDD ; ILRSLGEISA ; LTAKLSDLRR ; 500: HGKGDSPEAR ; ALAKQIATSL ; QNLQSKTNRA ; VANTRPVKAA ; VHLEGKIEQA ; 550: QRWIDNPTVD ; DRGVGQAAIR ; GLVAEGRRLA ; NVMMGPYRQD ; LLAKCDRVDQ ; 600: LAAQLADLAA ; RGEGESPQAR ; AIAAQLQDSL ; KDLKARMQEA ; MTQEVSDVFS ; 650: DTTTPIKLLA ; VAATAPSDTP ; NREEVFEERA ; ANFENHAARL ; GATAEKAAAV ; 700: GTANKTTVEG ; IQATVKSARE ; LTPQVVSAAR ; ILLRNPGNQA ; AYEHFETMKN ; 750: QWIDNVEKMT ; GLVDEAIDTK ; SLLDASEEAI ; KKDLDKCKVA ; MANMQPQMLV ; 800: AGATSIARRA ; NRILLVAKRE ; VENSEDPKFR ; EAVKAASDEL ; SKTISPMVMD ; 850: AKAVAGNISD ; PGLQKSFLDS ; GYRILGAVAK ; VREAFQPQEP ; DFPPPPPDLE ; 900: HLHLTDELAP ; PKPPLPEGEV ; PPPRPPPPEE ; KDEEFPEQKA ; GEAINQPMMM ; 950: AARQLHDEAR ; KWSSKPVTVI ; NEAAEAGVDI ; DEEDDADVEF ; SLPSDIEDDY ; 1000: EPELLLMPTN ; QPVNQPILAA ; AQSLHREATK ; WSSKGNDIIA ; AAKRMALLMA ; 1050: EMSRLVRGGS ; GNKRALIQCA ; KDIAKASDEV ; TRLAKEVAKQ ; CTDKRIRTNL ; 1100: LQVCERIPTI ; STQLKILSTV ; KATMLGRTNI ; SDEESEQATE ; MLVHNAQNLM ; 1135: QSVKETVREA ; EAASIKIRTD ; AGFTLRWVRK ; TPWYQ
Sequence Length	1135
String	P12003
Sub Unit	Exhibits self-association properties. Interacts with NRAP and TLN1. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin.

Uniprot	P12003
Gene Name	VCL
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:396422
Unigene	Gga.698

Reference	1. "cDNA-derived sequence of chicken embryo vinculin." Coutu M.D., Craig S.W. Proc. Natl. Acad. Sci. U.S.A.85:8535-8539(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) tissue: Embryo. 2. "Primary sequence and domain structure of chicken vinculin." Price G.J., Jones P., Davison M.D., Patel B., Bendori R., Geiger B., Critchley D.R. Biochem. J.259:453-461(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) tissue: Embryo. 3. "Chicken vinculin and meta-vinculin are derived from a single gene by alternative splicing of a 207-base pair exon unique to meta-vinculin." Byrne B.J., Kaczorowski Y.J., Coutu M.D., Craig S.W. J. Biol. Chem.267:12845-12850(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING (ISOFORM 2) 4. "Isolation and characterization of a vinculin cDNA from chick-embryo fibroblasts." Price G.J., Jones P., Davison M.D., Patel B., Eperon I.C., Critchley D.R. Biochem. J.245:595-603(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-881 tissue: Embryo. 5. "The cytoskeletal protein vinculin is acylated by myristic acid." Kellie S., Wigglesworth N.M. FEBS Lett.213:428-432(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION 6. "Identification of a talin binding site in the cytoskeletal protein vinculin." Jones P., Jackson P., Price G.J., Patel B., Ohanion V., Lear A.L., Critchley D.R. J. Cell Biol.109:2917-2927(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TALIN INTERACTION DOMAIN 7. "Molecular interactions of N-RAP, a nebulin-related protein of striated muscle myotendon junctions and intercalated disks." Luo G., Herrera A.H., Horowits R. Biochemistry38:6135-6143(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH NRAP 8. "The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading." Zhang Z., Izaguirre G., Lin S.-Y., Lee H.Y., Schaefer E., Haimovich B. Mol. Biol. Cell15:4234-4247(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-100 AND TYR-1134;FUNCTION;MUTAGENESIS OF TYR-100; TYR-160; TYR-537; TYR-692; TYR-822 AND TYR-1134 9. "Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner." le Duc Q., Shi Q., Blonk I., Sonnenberg A., Wang N., Leckband D., de Rooij J. J. Cell Biol.189:1107-1115(2010) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION 10. "Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin." Peng X., Cuff L.E., Lawton C.D., DeMali K.A. J. Cell Sci.123:567-577(2010) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH CTNNB1 11. "Crystal structure of the vinculin tail suggests a pathway for activation." Bakolitsa C., de Pereda J.M., Bagshaw C.R., Critchley D.R., Liddington R.C. Cell99:603-613(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 881-1135 OF ISOFORM 1 12. "RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover." Goult B.T., Zacharchenko T., Bate N., Tsang R., Hey F., Gingras A.R., Elliott P.R., Roberts G.C., Ballestrem C., Critchley D.R., Barsukov I.L. J. Biol. Chem.288:8238-8249(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-259 IN COMPLEX WITH APBB1IP;INTERACTION WITH TLN1 AND APBB1IP

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BHE13811844	E1839c	96T	$900		Add to Cart

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