JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Assay Kits / ELISA Kits

ELISA Kit for Chicken V(D)J recombination-activating protein 1

SKU : BHE13808823

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

EIAAB SCIENCE INC, WUHAN

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	E1550c
Categories	Elisa
Function	Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.
Specificity	Natural and recombinant Chicken V(D)J recombination-activating protein 1
Subcellular Location	Nucleus
Detection Range	0.312-20ng/ml
Entryname	RAG1_CHICK
Method Type	Sandwich
Platform	PF12940
Protein Name	V(D)J recombination-activating protein 1
Sensitivity	0.079ng/ml
Sequence	50: MSITSRMDLP ; EEIQHPYAKF ; SEWKFKLFKV ; RPFQKEPSDK ; SQCINKDQEQ ; 100: EVASTDKNIT ; LHKDEEVPRG ; EKLILTQKDF ; MGNTQALEKD ; VNDMKIQDNT ; 150: AHQNNLKQLC ; RICGVSFKTD ; CYKRTHPVHG ; PVDDETLWLL ; RKKEKKATSW ; 200: PDLIAKVFKI ; DVRGDVDTIH ; PTRFCHNCWS ; IIHRKFSNTP ; CEVYFPRNST ; 250: MEWQPHSANC ; EVCHTPSRGV ; KRKSQPPNVQ ; HGKRVKIIAE ; RARVNRGIKN ; 300: QVIKNKNVMK ; EITNCKNRHL ; STKLLAVDYP ; ADFIKSISCQ ; ICEHILADPV ; 350: ETTCRHLFCR ; TCILSCIRVM ; GCYCPSCWYP ; CFPTDLVTPV ; KSFLNILDSL ; 400: SIRCPVPECD ; EEILHGKYGQ ; HFSNHKEMKD ; KELYNPINKG ; GRPRQHLLSL ; 450: TRRAQKHRLR ; ELKRQVKAFA ; EKEEGGDIKA ; VCMTLFLLAL ; RAKNEHKQAD ; 500: ELEAIMQGRG ; SGLHPAVCLA ; IRINTFLSCS ; QYHKMYRTVK ; AVTGRQIFQP ; 550: LHALRTAEKA ; LLPGYHPFEW ; KPPLKNVSTN ; TEVGIIDGLS ; GLPLSIDDYP ; 600: IDTIAKRFRY ; DTALVSALKD ; MEEEILEGMK ; AKNLDDYLNG ; PFTVVVKECC ; 650: DGMGDVSEKH ; GSGPAVPEKA ; VRFSFTVMNI ; AIDHENERIR ; IFEEVKPNSE ; 700: LCCKPLCLML ; ADESDHETLT ; AILSPLIAER ; EAMKNSELLL ; EIGGILRTFK ; 750: FIFRGTGYDE ; KLVREVEGLE ; ASGSTYICTL ; CDATRLEASQ ; NLVFHSITRS ; 800: HAENLERYEI ; WRSNPYHESV ; DELRDRVKGV ; SAKPFIETVP ; SIDALHCDIG ; 850: NATEFYRIFQ ; MEIGEVYKNP ; DATKEERKRW ; QLTLDKHLRK ; KMKLKPMMRM ; 900: SGNFARKLMS ; KETVEAVCEL ; IKCEERHEAL ; KELMDLYLKM ; KPVWRSSCPA ; 950: KECPELLCQY ; SYNSQRFAEL ; LSTKFKYRYE ; GKITNYFHKT ; LAHVPEIIER ; 1000: DGSIGAWASE ; GNESGNKLFR ; RFRKMNARQS ; KFYEMEDVLK ; HHWLYTSKYL ; 1041: QKFMNAHKTL ; RSQGFAIDPD ; DGLGDSLPPE ; ISLESNDSVE ; L
Sequence Length	1041
String	P24271
Sub Unit	Homodimer. Component of the RAG complex composed of core components RAG1 and RAG2.

Uniprot	P24271
Gene Name	RAG1
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:423164
Unigene	Gga.52953

Reference	1. "Selective expression of RAG-2 in chicken B cells undergoing immunoglobulin gene conversion." Carlson L.M., Oettinger M.A., Schatz D.G., Masteller E.L., Hurley E.A., McCormack W.I., Baltimore D., Thompson C.B. Cell64:201-208(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13808823	E1550c	96T	$900		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.