ELISA Kit for Chicken Bone morphogenetic protein 2

Mfr.No.

E0013c

Alternative Names

BMP-2

Categories

Elisa

Function

Negatively regulates the structure and function of the limb apical ectodermal ridge.

Specificity

Natural and recombinant Chicken Bone morphogenetic protein 2

Subcellular Location

Secreted

Detection Range

0.156-10ng/mL

Entryname

BMP2_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-105%	96-101%	99-105%	103-106%
EDTA plasma(n=5)	99-105%	102-104%	95-99%	98-104%
heparin plasma(n=5)	100-106%	92-97%	101-104%	106-110%

Method Type

Sandwich

Platform

PF00019

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.6%

Protein Name

Bone morphogenetic protein 2

Recovery

Recovery was determined by spiking various levels of BMP2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	98-103
Plasma	100	99-106

Sensitivity

0.078ng/mL

Sequence

50:

GSLKRPEDLL ; GEFELRLLHM ; FGLKRRPSPG ; KDVVIPPYML ; DLYRLHAGQQ ;

100:

LGYPLERAAC ; RANTVCSFHH ; EEVLEELPET ; SGKTARRFFF ; NLTSIPNEES ;

150:

VTSAELQIFP ; GEQVHEAFES ; NSSYHHRINI ; YEIMKPATAT ; SKDPVTRLLD ;

200:

TRLVHHNASK ; WESSDVTPAV ; LRWIAHGQPN ; HGFVVEVVHL ; DKENSASKRH ;

250:

VRISRSLHQD ; EDSWSQLRPL ; LVTFGHDGKG ; HPLHKREKRQ ; AKHKQRKRHK ;

300:

YSCKRHPLYV ; DFNDVGWNDW ; IVAPPGYSAF ; YCHGECPFPL ; ADHLNSTNHA ;

350:

IVQTLVNSVN ; SKIPKACCVP ; TELSAISMLY ; LDENEKVVLK ; NYQDMVVEGC ;

353:

GCR

Sequence Length

353

String

Q90751

Sub Unit

Homodimer; disulfide-linked.

Uniprot	Q90751
Gene Name	BMP2
Immunogen Species	Gallus Gallus (Chicken)
Unigene	Gga.3950

Reference	1. "Bone morphogenetic proteins and a signalling pathway that controls patterning in the developing chick limb." Francis P.H., Richardson M.K., Brickell P.M., Tickle C. Development120:209-218(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: White leghorn. 2. "BMPs negatively regulate structure and function of the limb apical ectodermal ridge." Pizette S., Niswander L. Development126:883-894(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION

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SKU	Supplier No.	Size	Your price	Quantity
BHE13801706	E0013c	96T	$900		Add to Cart

ELISA Kit for Chicken Bone morphogenetic protein 2

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