ELISA Kit for Chicken Alpha-N-acetylgalactosaminidase

Mfr.No.

E13422c

Alternative Names

Alpha-galactosidase B

Categories

Elisa

Function

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.

Specificity

Natural and recombinant Chicken Alpha-N-acetylgalactosaminidase

Subcellular Location

Lysosome

Entryname

NAGAB_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-102%	91-103%	95-106%	92-107%
EDTA plasma(n=5)	89-97%	93-104%	95-105%	93-106%
heparin plasma(n=5)	93-102%	94-107%	101-108%	97-109%

Method Type

Sandwich

Platform

PF02065

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.3%

Protein Name

Alpha-N-acetylgalactosaminidase

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-105
Plasma	98	93-104

Sequence

50:

LENGLARTPP ; MGWLAWERFR ; CNVNCREDPR ; QCISEMLFME ; MADRIAEDGW ;

100:

RELGYKYINI ; DDCWAAKQRD ; AEGRLVPDPE ; RFPRGIKALA ; DYVHARGLKL ;

150:

DIYGDLGRLT ; CGGYPGTTLD ; RVEQDAQTFA ; EWGVDMLKLD ; GCYSSGKEQA ;

200:

QGYPQMARAL ; NSTGRPIVYS ; CSWPAYQGGL ; PPKVNYTLLG ; EICNLWRNYD ;

250:

DIQDSWDSVL ; SIVDWFFTNQ ; DVLQPFAGPG ; HWNDPDMLII ; GNFGLSYEQS ;

300:

RSQMALWTIM ; AAPLLMSTDL ; RTISPSAKKI ; LQNRLMIQIN ; QDPLGIQGRR ;

350:

IIKEGSHIEV ; FLRPLSQAAS ; ALVFFSRRTD ; MPFRYTTSLA ; KLGFPMGAAY ;

400:

EVQDVYSGKI ; ISGLKTGDNF ; TVIINPSGVV ; MWYLCPKALL ; IQQQAPGGPS ;

405:

RLPLL

Sequence Length

405

String

Q90744

Sub Unit

Homodimer.

Uniprot	Q90744
Gene Name	NAGA
Immunogen Species	Gallus Gallus (Chicken)
Unigene	Gga.4369

Reference	1. "Cloning and sequence of a chicken alpha-N-acetylgalactosamindase gene." Davis M.O., Hata J., Smith D., Walker J.C. Biochim. Biophys. Acta1216:296-298(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE tissue: Liver. 2. "The 1.9 A structure of alpha-N-acetylgalactosaminidase: molecular basis of glycosidase deficiency diseases." Garman S.C., Hannick L., Zhu A., Garboczi D.N. Structure10:425-434(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH N-ACETYLGALACTOSAMINE;GLYCOSYLATION AT ASN-161; ASN-185 AND ASN-369;SUBUNIT;DISULFIDE BONDS

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SKU	Supplier No.	Size	Your price	Quantity
BHE13806797	E13422c	96T	$900		Add to Cart

ELISA Kit for Chicken Alpha-N-acetylgalactosaminidase

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