Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
Specificity
Natural and recombinant Bovine Retinoid isomerohydrolase
Subcellular Location
Cytoplasm Cell membrane Lipid-anchor Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 98-118;PALMITOYLATION;IDENTIFICATION BY MASS SPECTROMETRY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION AT SER-2;PALMITOYLATION AT CYS-231; CYS-329 AND CYS-330;IDENTIFICATION BY MASS SPECTROMETRY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CATALYTIC ACTIVITY;FUNCTION;BIOPHYSICOCHEMICAL PROPERTIES
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.14 ANGSTROMS) IN COMPLEX WITH IRON ION;CATALYTIC ACTIVITY;FUNCTION;SUBCELLULAR LOCATION;PALMITOYLATION AT CYS-112;IDENTIFICATION BY MASS SPECTROMETRY;COFACTOR;TISSUE SPECIFICITY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH IRON ION;CATALYTIC ACTIVITY;FUNCTION;SUBCELLULAR LOCATION;COFACTOR;TISSUE SPECIFICITY
Reviews of ELISA Kit for Bovine Retinoid isomerohydrolase