ELISA Kit for Bovine Polymeric immunoglobulin receptor

Mfr.No.

E1286b

Categories

Elisa

Function

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Specificity

Natural and recombinant Bovine Polymeric immunoglobulin receptor

Subcellular Location

Secretory component Secreted

Entryname

PIGR_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIgR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	85-98%	80-96%	93-101%	93-105%
EDTA plasma(n=5)	90-101%	85-94%	89-96%	87-99%
heparin plasma(n=5)	83-94%	90-102%	94-103%	92-101%

Platform

PF07686

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.5

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1

Protein Name

Polymeric immunoglobulin receptor

Recovery

Recovery was determined by spiking various levels of PIgR into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	88	79-99
Plasma	95	90-103

Sequence

50:

MSRLFLACLL ; AIFPVVSMKS ; PIFGPEEVTS ; VEGRSVSIKC ; YYPPTSVNRH ;

100:

TRKYWCRQGA ; QGRCTTLISS ; EGYVSDDYVG ; RANLTNFPES ; GTFVVDISHL ;

150:

THKDSGRYKC ; GLGISSRGLN ; FDVSLEVSQD ; PAQASHAHVY ; TVDLGRTVTI ;

200:

NCPFTRANSE ; KRKSLCKKTI ; QDCFQVVDST ; GYVSNSYKDR ; AHISILGTNT ;

250:

LVFSVVINRV ; KLSDAGMYVC ; QAGDDAKADK ; INIDLQVLEP ; EPELVYGDLR ;

300:

SSVTFDCSLG ; PEVANVPKFL ; CQKKNGGACN ; VVINTLGKKA ; QDFQGRIVSV ;

350:

PKDNGVFSVH ; ITSLRKEDAG ; RYVCGAQPEG ; EPQDGWPVQA ; WQLFVNEETA ;

400:

IPASPSVVKG ; VRGGSVTVSC ; PYNPKDANSA ; KYWCHWEEAQ ; NGRCPRLVES ;

450:

RGLIKEQYEG ; RLALLTEPGN ; GTYTVILNQL ; TDQDTGFYWC ; VTDGDTRWIS ;

500:

TVELKVVQGE ; PSLKVPKNVT ; AWLGEPLKLS ; CHFPCKFYSF ; EKYWCKWSNR ;

550:

GCSALPTQND ; GPSQAFVSCD ; QNSQVVSLNL ; DTVTKEDEGW ; YWCGVKEGPR ;

600:

YGETAAVYVA ; VESRVKGSQG ; AKQVKAAPAG ; AAIQSRAGEI ; QNKALLDPSF ;

650:

FAKESVKDAA ; GGPGAPADPG ; RPTGYSGSSK ; ALVSTLVPLA ; LVLVAGVVAI ;

700:

GVVRARHRKN ; VDRISIRSYR ; TDISMSDFEN ; SRDFEGRDNM ; GASPEAQETS ;

750:

LGGKDEFATT ; TEDTVESKEP ; KKAKRSSKEE ; ADEAFTTFLL ; QAKNLASAAT ;

757:

QNGPTEA

Sequence Length

757

String

P81265

Uniprot	P81265
Gene Name	PIGR
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:281401
Unigene	Bt.4695

Reference	1. "Cloning and characterization of two forms of bovine polymeric immunoglobulin receptor cDNA." Kulseth M.A., Krajci P., Myklebost O., Rogne S. DNA Cell Biol.14:251-256(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];ALTERNATIVE SPLICING tissue: Mammary gland. tissue: Small intestine. 2. "Cloning and characterization of the bovine polymeric immunoglobulin receptor-encoding cDNA." Verbeet M.P., Vermeer H., Warmerdam G.C., de Boer H.A., Lee S.H. Gene164:329-333(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Mammary gland.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808850	E1286b	96T	$900		Add to Cart

ELISA Kit for Bovine Polymeric immunoglobulin receptor

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