ELISA Kit for Bovine PDZ and LIM domain protein 1

Mfr.No.

E3134b

Alternative Names

Elfin

Categories

Elisa

Function

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.

Specificity

Natural and recombinant Bovine PDZ and LIM domain protein 1

Subcellular Location

Cytoplasm Cytoplasm cytoskeleton Associates with the actin stress fibers.

Entryname

PDLI1_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-102%	93-104%	95-106%	90-103%
EDTA plasma(n=5)	89-94%	94-101%	91-97%	92-104%
heparin plasma(n=5)	91-102%	90-106%	94-108%	93-105%

Platform

PF00412

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 7.5%

Protein Name

PDZ and LIM domain protein 1

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-105
Plasma	96	94-103

Sequence

50:

MTTLQIVLQG ; PGPWGFRLVG ; GKDFEQPLAI ; SRVTPGSKAA ; IGNLCVGDVI ;

100:

TAIDGENTSN ; MTHLEAQNKI ; KGCTDNMTLT ; VARSEQKIWS ; PLVTEEGKRH ;

150:

PYKMNLASEP ; QEALHIGSAH ; NRSAVPFTAS ; PAFSSAPRVI ; TNQYNNPAGL ;

200:

YSSENISSFN ; NALESKTAAS ; GQENGRALDH ; SQLPSGLVID ; KESEVYKMLQ ;

250:

EKQELNEPPK ; QSTSFLVLQE ; ILESEEKGDP ; NKPSGFRSVK ; APVTKVAASI ;

300:

GNAQKLPMCD ; KCGTGIVGVF ; VKLRERHRHP ; ECYVCTDCGT ; NLKQKGHFFV ;

328:

EDQIYCEKHA ; RERVTPPEGY ; DVITVFPK

Sequence Length

328

Sub Unit

Interacts with alpha-actinins 1, 2 and 4.

Uniprot	Q5E9E1
Gene Name	PDLIM1
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:614675
Unigene	Bt.32810

Reference	1. "Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics6:166-166(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809038	E3134b	96T	$900		Add to Cart

ELISA Kit for Bovine PDZ and LIM domain protein 1

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