ELISA Kit for Bovine Major prion protein

Mfr.No.

E9345b

Alternative Names

Major scrapie-associated fibril protein 1 ; CD230 ; PRP

Categories

Elisa

Function

The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).

Specificity

Natural and recombinant Bovine Major prion protein

Subcellular Location

Cell membrane Lipid-anchor GPI-anchor Golgi apparatus

Detection Range

0.156-10ng/mL

Entryname

PRIO_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-102%	92-103%	95-105%	92-107%
EDTA plasma(n=5)	88-96%	93-101%	94-102%	95-106%
heparin plasma(n=5)	93-104%	94-105%	101-106%	95-109%

Method Type

Sandwich

Platform

PF00377

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.6%

Protein Name

Major prion protein

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-106
Plasma	98	92-108

Sensitivity

0.075 ng/mL

Sequence

50:

MVKSHIGSWI ; LVLFVAMWSD ; VGLCKKRPKP ; GGGWNTGGSR ; YPGQGSPGGN ;

100:

RYPPQGGGGW ; GQPHGGGWGQ ; PHGGGWGQPH ; GGGWGQPHGG ; GWGQPHGGGG ;

150:

WGQGGTHGQW ; NKPSKPKTNM ; KHVAGAAAAG ; AVVGGLGGYM ; LGSAMSRPLI ;

200:

HFGSDYEDRY ; YRENMHRYPN ; QVYYRPVDQY ; SNQNNFVHDC ; VNITVKEHTV ;

250:

TTTTKGENFT ; ETDIKMMERV ; VEQMCITQYQ ; RESQAYYQRG ; ASVILFSSPP ;

264:

VILLISFLIF ; LIVG

Sequence Length

264

String

P10279

Sub Unit

Monomer and homodimer. Has a tendency to aggregate into amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Soluble oligomers may represent an intermediate stage on the path to fibril formation. Copper binding may promote oligomerization. Interacts with APP, GRB2, ERI3/PRNPIP and SYN1. Mislocalized cytosolically exposed PrP interacts with MGRN1; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement.

Uniprot	P10279
Gene Name	PRNP
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:281427
Unigene	Bt.4737

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BHE13809218	E9345b	96T	$900		Add to Cart

ELISA Kit for Bovine Major prion protein

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