IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Specificity
Natural and recombinant Bovine Interleukin-8
Subcellular Location
Secreted
Detection Range
31.2-2000pg/mL
Entryname
IL8_BOVIN
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
Protein Name
Interleukin-8
Recovery
Recovery was determined by spiking various levels of IL8 into serum and plasma .
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2.
"Cloning and sequencing of bovine interleukin 8 cDNA isolated from lipopolysaccharide stimulated monocytes in vitro." Galligan C.L., Yoshimura T., Coomber B.L. Submitted (2000-02) to the EMBL/GenBank/DDBJ databases
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