ELISA Kit for Bovine Interleukin-10

Mfr.No.

E0056b

Alternative Names

Cytokine synthesis inhibitory factor ; CSIF

Categories

Elisa

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Specificity

Natural and recombinant Bovine Interleukin-10

Subcellular Location

Secreted

Detection Range

31.2-2000pg/mL

Entryname

IL10_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	98-103%	107-111%	90-98%
EDTA plasma(n=5)	99-106%	103-105%	97-103%	101-104%
heparin plasma(n=5)	102-104%	90-98%	105-110%	87-95%

Method Type

Sandwich

Platform

PF00726

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.3%

Protein Name

Interleukin-10

Recovery

Recovery was determined by spiking various levels of IL10 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	99-105
Plasma	103	102-108

Sensitivity

13.6pg/mL

Sequence

50:

MHSSALLCCL ; VFLAGVAASR ; DASTLSDSSC ; IHLPTSLPHM ; LRELRAAFGK ;

100:

VKTFFQMKDQ ; LHSLLLTQSL ; LDDFKGYLGC ; QALSEMIQFY ; LEEVMPQAEN ;

150:

HGPDIKEHVN ; SLGEKLKTLR ; LRLRRCHRFL ; PCENKSKAVE ; KVKRVFSELQ ;

178:

ERGVYKAMSE ; FDIFINYIET ; YMTTKMQK

Sequence Length

178

String

P43480

Sub Unit

Homodimer.

Uniprot	P43480
Gene Name	IL10
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:281246
Unigene	Bt.4723

Reference	1. "Characterization of a cDNA encoding bovine interleukin 10: kinetics of expression in bovine lymphocytes." Hash S.M., Brown W.C., Rice-Ficht A.C. Gene139:257-261(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Charolais. tissue: Blood. 2. "U.S. veterinary immune reagent network: expressed bovine gene sequences." U.S. Veterinary Immune Reagent Network, Hudgens T., Tompkins D., Baldwin C.L. Submitted (2007-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Belted Galloway. tissue: Peripheral blood.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13806042	E0056b	96T	$900		Add to Cart

ELISA Kit for Bovine Interleukin-10

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