ELISA Kit for Bovine Erythropoietin

Mfr.No.

E0028b

Categories

Elisa

Function

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Specificity

Natural and recombinant Bovine Erythropoietin

Subcellular Location

Secreted

Detection Range

78-5000pg/ml

Entryname

EPO_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-100%	90-98%	95-102%	92-97%
EDTA plasma(n=5)	89-95%	93-98%	99-105%	90-97%
heparin plasma(n=5)	93-98%	99-105%	101-107%	96-105%

Method Type

Sandwich

Platform

PF00758

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

Erythropoietin

Recovery

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-99
Plasma	98	97-102

Sensitivity

35pg/ml

Sequence

50:

MGARDCTPLL ; MLSFLLFPLG ; FPVLGAPARL ; ICDSRVLERY ; ILEAREAENA ;

100:

TMGCAEGCSF ; NENITVPDTK ; VNFYAWKRME ; VQQQALEVWQ ; GLALLSEAIL ;

150:

RGQALLANAS ; QPCEALRLHV ; DKAVSGLRSL ; TSLLRALGAQ ; KEAISLPDAT ;

192:

PSAAPLRAFT ; VDALSKLFRI ; YSNFLRGKLT ; LYTGEACRRG ; DR

Sequence Length

192

String

P48617

Uniprot	P48617
Gene Name	EPO
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:280784
Unigene	Bt.525

Reference	1. "Cloning of a cDNA encoding bovine erythropoietin and analysis of its transcription in selected tissues." Suliman H.B., Majiwa P.A.O., Feldman B.F., Mertens B., Logan-Henfrey L.L. Gene171:275-280(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Boran. tissue: Kidney. 2. "Cloning and sequence analysis on erythropoietin (EPO) gene of Bos taurus (San Jiang yellow cattle)." Jin S., Zhong J.C., Chen Z.H., Ma Z.J. Submitted (2007-01) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

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SKU	Supplier No.	Size	Your price	Quantity
BHE13803526	E0028b	96T	$900		Add to Cart

ELISA Kit for Bovine Erythropoietin

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