ELISA Kit for Bovine DNA mismatch repair protein Msh2

Mfr.No.

E8706b

Alternative Names

MutS protein homolog 2

Categories

Elisa

Function

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Specificity

Natural and recombinant Bovine DNA mismatch repair protein Msh2

Subcellular Location

Nucleus

Entryname

MSH2_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MSH2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-106%	94-109%	95-106%	92-110%
EDTA plasma(n=5)	90-108%	91-106%	92-107%	94-105%
heparin plasma(n=5)	93-106%	95-104%	93-105%	93-107%

Platform

PF01624

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.7%

Protein Name

DNA mismatch repair protein Msh2

Recovery

Recovery was determined by spiking various levels of MSH2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	90-106
Plasma	99	92-105

Sequence

50:

MAVQPKDTLQ ; LDSAAEVGFV ; RFFQGMPEKP ; TTTVRLFDRG ; DFYTAHREDA ;

100:

LLAAREVFKT ; QGVVKYMGPA ; GAKTLESVVL ; SKMNFESFVK ; DLLLVRQYRV ;

150:

EVYKNRAGNK ; ASKENDWYLA ; FKASPGNLSQ ; FEDILFGNND ; MSASIGVVGV ;

200:

KMSTVDGQRQ ; VGVGYVDSTQ ; RKLGLCEFPD ; NDQFSNLEAL ; LIQIGPKECV ;

250:

MPGGETAGDM ; GKLRQVIQRG ; GILITERKRA ; DFSTKDIYQD ; LNRLLKGKKG ;

300:

EQVNSAVLPE ; MENQVAVSSL ; SAVIKFLELL ; SDDSNFGQFE ; LTTFDFSQYM ;

350:

KLDIAAVRAL ; NLFQGSVEDT ; SGSQSLAALL ; NKCKTPQGQR ; LVNQWIKQPL ;

400:

MDKNRIEERL ; NLVEAFVEDA ; ELRQNLQEDL ; LRRFPDLNRL ; AKKFQRQAAN ;

450:

LQDCYRLYQG ; INQLPNVIQA ; LEKYEGKHQA ; LFLAVFVTPL ; IDLRSDFSKF ;

500:

QEMIETTLDM ; DQVENHEFLV ; KPSFDPNLSE ; LREIMDDLEK ; KMQSTLVSAA ;

550:

RDLGLDPGKQ ; IKLDSSTQFG ; YYFRVTCKEE ; KVLRNNKNFS ; TVDIQKNGVK ;

600:

FTNSKLTSLN ; EEYTKNKTEY ; EEAQNAIVKE ; IVNISSGYVE ; PMQTLNDVLA ;

650:

QLDAVVSFAH ; VSDAAPVPYV ; RPVILEKGRG ; RITLKASRHA ; CVEVQDEVAF ;

700:

IPNDVHFEKD ; KQMFHIITGP ; NMGGKSTYIR ; QTGVVVLMAQ ; IGCFVPCEWA ;

750:

EVSIVDCILA ; RVGAGDSQLK ; GVSTFMAEML ; ETASILRSAT ; KDSLIIIDEL ;

800:

GRGTSTYDGF ; GLAWAISEYI ; ATKIGAFCMF ; ATHFHELTAL ; ANQIPTVNNL ;

850:

HVTALTTEET ; LTMLYQVKKG ; VCDQSFGIHV ; AELANFPRHV ; IECAKQKALE ;

900:

LEEFQNIGKP ; QECDEMEPAA ; KRCYLEREQG ; EKIIQEFLSK ; VKQVPFTEMS ;

934:

EESITRKLKQ ; LKAEVIAKNN ; SFVNEIISRI ; KVTA

Sequence Length

934

String

Q3MHE4

Sub Unit

Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2-MSH3 (MutS beta). Both heterodimer form a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases.

Uniprot	Q3MHE4
Gene Name	MSH2
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:533115
Unigene	Bt.635

Reference	1. NIH - Mammalian Gene Collection (MGC) project Submitted (2005-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Hereford. tissue: Ascending colon.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13806789	E8706b	96T	$900		Add to Cart

ELISA Kit for Bovine DNA mismatch repair protein Msh2

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