ELISA Kit for Bovine Cadherin-1

Mfr.No.

E0017b

Alternative Names

Epithelial cadherin ; E-cadherin ; CD324

Categories

Elisa

Function

E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Specificity

Natural and recombinant Bovine Cadherin-1

Subcellular Location

Cell junction Cell membrane Single-pass type I membrane protein Endosome Golgi apparatus trans-Golgi network Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm (By similarity). Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Detection Range

0.156-10ng/mL

Entryname

CADH1_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-105%	94-103%	95-105%	92-108%
EDTA plasma(n=5)	89-98%	93-101%	90-99%	90-104%
heparin plasma(n=5)	93-101%	92-102%	99-109%	94-107%

Method Type

Sandwich

Platform

PF00028

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 11.2%

Protein Name

Cadherin-1

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	96-105
Plasma	104	94-106

Sensitivity

0.078ng/mL

Sequence

50:

MGPWSRSLSA ; LCCCCRCNPW ; LCREPEPCIP ; GFGAESYTFT ; VPRRNLERGR ;

100:

VLGRVSFEGC ; AGLPRTVYVS ; DDTRFKVHTD ; GVLTVRRPVH ; LHRPELSFLV ;

150:

HAWDSTHRKL ; STKVTLEVSA ; HHHHHHSHHD ; SPSGTQTEVL ; TFPGPHHGLR ;

200:

RQKRDWVIPP ; ISCPENEKGP ; FPKSLVQIKS ; NKEKETQVFY ; SITGQRADTP ;

250:

PVGVFIIERE ; TGWLKVTQPL ; DREQIAKYIL ; FSHAVSSNGQ ; AIEEPMEIVI ;

300:

TVTDQNDNKP ; QFTQEVFKAS ; ALEGALPGTS ; VMQVTATDID ; DEVNTYTAAI ;

350:

GYTIPAQDPM ; LPHNKMFTIN ; KETGVISVLT ; TGLDRESFPT ; YTLMVQAADL ;

400:

NGEGLSTTAT ; AVITVLDTND ; NAPRFNPTTY ; VGSVPENEAN ; VAITTLTVTD ;

450:

ADDPNTPAWE ; AVYTVLNDNE ; KQFIVVTDPV ; TNEGTLKTAK ; GLDFEAKQQY ;

500:

ILYVAVTNVA ; PFEVTLPTST ; ATVTVDVIDV ; NEAPIFVPPQ ; KRVEVPEDFG ;

550:

VGLEITSYTA ; REPDTFMEQK ; ITYRIWRDTA ; NWLEINPETG ; AISTRAELDR ;

600:

EDVDHVKNST ; YTALIIATDN ; GSPPATGTGT ; LLLFLDDVND ; NGPVPEPRTM ;

650:

DFCQRNPEPH ; IININDPDLP ; PNTSPFTAEL ; THGASVNWTI ; EYNDQERESL ;

700:

ILKPKKTLEL ; GDHKINLKLI ; DNQNKDQVTT ; LDVHVCDCDG ; IVSNCRKARP ;

750:

AEAGLQVPAI ; LGILGGILAF ; LILILLLLLL ; VRRRRVVKEP ; LLPPEDDTRD ;

800:

NVYYYDEEGG ; GEEDQDFDLS ; QLHRGLDARP ; EVTRNDVAPT ; LMSVPQYRPR ;

850:

PANPDEIGNF ; IDENLKAADS ; DPTAPPYDSL ; LVFDYEGSGS ; EAATLSSLNS ;

882:

SESDQDQDYD ; YLNEWGNRFK ; KLADMYGGGE ; DD

Sequence Length

882

String

Q6R8F2

Sub Unit

Homodimer; disulfide-linked. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1, beta-catenin/CTNNB1 or gamma-catenin/JUP, and potentially alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5 (By similarity). Interacts with TBC1D2. Interacts with CAV1. Interacts with the TRPV4 and CTNNB1 complex.

Uniprot	Q6R8F2
Gene Name	CDH1
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:282637
Unigene	Bt.64827

Reference	1. "Sequence of Bos taurus e-cadherin (CDH1) mRNA." Hanna C.B., Cox M.L., Golding M.C., Westhusin M.E., Kraemer D.C. Submitted (2003-12) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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BHE13801589	E0017b	96T	$900		Add to Cart

ELISA Kit for Bovine Cadherin-1

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