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ELISA Kit for Rat Glycogen synthase kinase-3 beta

SKU : BHE13804808

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E2298r
Alternative Names	Factor A ; FA ; Serine/threonine-protein kinase GSK3B ; 2.7.11.1
Categories	Elisa
Function	Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity.
Specificity	Natural and recombinant Rat Glycogen synthase kinase-3 beta
Subcellular Location	Cytoplasm Nucleus Membrane Cell membrane The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
Detection Range	0.312-20ng/mL
Entryname	GSK3B_RAT
Method Type	Sandwich
Platform	PF00069
Protein Name	Glycogen synthase kinase-3 beta
Sensitivity	0.172ng/mL
Sequence	50: MSGRPRTTSF ; AESCKPVQQP ; SAFGSMKVSR ; DKDGSKVTTV ; VATPGQGPDR ; 100: PQEVSYTDTK ; VIGNGSFGVV ; YQAKLCDSGE ; LVAIKKVLQD ; KRFKNRELQI ; 150: MRKLDHCNIV ; RLRYFFYSSG ; EKKDEVYLNL ; VLDYVPETVY ; RVARHYSRAK ; 200: QTLPVIYVKL ; YMYQLFRSLA ; YIHSFGICHR ; DIKPQNLLLD ; PDTAVLKLCD ; 250: FGSAKQLVRG ; EPNVSYICSR ; YYRAPELIFG ; ATDYTSSIDM ; WSAGCVLAEL ; 300: LLGQPIFPGD ; SGVDQLVEII ; KVLGTPTREQ ; IREMNPNYTE ; FKFPQIKAHP ; 350: WTKVFRPRTP ; PEAIALCSRL ; LEYTPTARLT ; PLEACAHSFF ; DELRDPNVKL ; 400: PNGRDTPALF ; NFTTQELSSN ; PPLATILIPP ; HARIQAAASP ; PANATAASDT ; 420: NAGDRGQTNN ; AASASASNST ;
Sequence Length	420
String	P18266
Sub Unit	Monomer (By similarity). Interacts with ARRB2, AXIN1, CABYR, DISC1, MMP2, MUC1, NIN and PRUNE (By similarity). Interacts with AXIN1; the interaction mediates hyperphosphorylation of CTNNB1 leading to its ubiquitination and destruction. Interacts with and phosphorylates SNAI1. Interacts with DNM1L (via a C-terminal domain) (By similarity). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B.

Uniprot	P18266
Gene Name	Gsk3b
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:84027
Unigene	Rn.10426

Reference	1. "Molecular cloning and expression of glycogen synthase kinase-3/factor A." Woodgett J.R. EMBO J.9:2431-2438(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Brain. 2. "Glycogen synthase kinase 3 beta is identical to tau protein kinase I generating several epitopes of paired helical filaments." Ishiguro K., Shiratsuchi A., Sato S., Omori A., Arioka M., Kobayashi S., Uchida T., Imahori K. FEBS Lett.325:167-172(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Brain cortex. 3. "Modulation of the glycogen synthase kinase-3 family by tyrosine phosphorylation." Hughes K., Nikolakaki E., Plyte S.E., Totty N.F., Woodgett J.R. EMBO J.12:803-808(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-216;MUTAGENESIS OF TYR-216 4. "Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin." Ikeda S., Kishida S., Yamamoto H., Murai H., Koyama S., Kikuchi A. EMBO J.17:1371-1384(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH CTNNB1/BETA-CATENIN 5. "The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathway." Chen H.J., Lin C.M., Lin C.S., Perez-Olle R., Leung C.L., Liem R.K. Genes Dev.20:1933-1945(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;IDENTIFICATION IN A COMPLEX WITH MACF1; APC; AXIN1 AND CTNNB1 6. "Inhibition of GSK3 promotes replication and survival of pancreatic beta cells." Mussmann R., Geese M., Harder F., Kegel S., Andag U., Lomow A., Burk U., Onichtchouk D., Dohrmann C., Austen M. J. Biol. Chem.282:12030-12037(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN REGULATION OF PANCREATIC BETA-CELLS 7. "Glycogen synthase kinase (GSK) 3beta directly phosphorylates Serine 212 in the regulatory loop and inhibits microtubule affinity-regulating kinase (MARK) 2." Timm T., Balusamy K., Li X., Biernat J., Mandelkow E., Mandelkow E.M. J. Biol. Chem.283:18873-18882(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF MARK2;MUTAGENESIS OF SER-9

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