ELISA Kit for Rat 14-3-3 protein theta

Mfr.No.

E14293r

Alternative Names

14-3-3 protein tau

Categories

Elisa

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Specificity

Natural and recombinant Rat 14-3-3 protein theta

Subcellular Location

Cytoplasm

Detection Range

0.312-20ng/mL

Entryname

1433T_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein theta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-106%	95-102%	90-110%	96-104%
EDTA plasma(n=5)	91-104%	93-103%	92-106%	93-103%
heparin plasma(n=5)	94-101%	91-107%	96-101%	92-105%

Method Type

Sandwich

Platform

PF00244

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

Protein Name

14-3-3 protein theta

Recovery

Recovery was determined by spiking various levels of 14-3-3 protein theta into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	94-103
Plasma	98	92-101

Sensitivity

0.156ng/mL

Sequence

50:

MEKTELIQKA ; KLAEQAERYD ; DMATCMKAVT ; EQGAELSNEE ; RNLLSVAYKN ;

100:

VVGGRRSAWR ; VISSIEQKTD ; TSDKKLQLIK ; DYREKVESEL ; RSICTTVLEL ;

150:

LDKYLIANAT ; NPESKVFYLK ; MKGDYFRYLA ; EVACGDDRKQ ; TIENSQGAYQ ;

200:

EAFDISKKEM ; QPTHPIRLGL ; ALNFSVFYYE ; ILNNPELACT ; LAKTAFDEAI ;

245:

AELDTLNEDS ; YKDSTLIMQL ; LRDNLTLWTS ; DSAGEECDAA ; EGAEN

Sequence Length

245

String

P68255

Sub Unit

Homodimer (By similarity) Interacts with CDK16. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 (By similarity). Interacts with GAB2.

Uniprot	P68255
Gene Name	Ywhaq
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:25577
Unigene	Rn.2502

Reference	1. "Molecular cloning of rat cDNAs for the zeta and theta subtypes of 14-3-3 protein and differential distributions of their mRNAs in the brain." Watanabe M., Isobe T., Ichimura T., Kuwano R., Takahashi Y., Kondo H., Inoue Y. Brain Res. Mol. Brain Res.25:113-121(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Wistar. tissue: Brain. 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Prostate. 3. Lubec G., Kang S.U., Chen W.-Q. Submitted (2009-01) to the UniProtKB [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 4-9; 12-18; 28-49; 61-68; 84-115; 128-157; 159-167 AND 194-222;IDENTIFICATION BY MASS SPECTROMETRY strain: Sprague-Dawley. tissue: Brain. tissue: Hippocampus.

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ELISA Kit for Rat 14-3-3 protein theta

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