Rat Fibroblast Growth Factor 2, Basic (FGF2) (Competitive EIA) ELISA Kit

Mfr.No.

CEA551Ra

Description

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2).
No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.

Alternative Names

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

Reactivity

Rat

Detection Method

Colormetric

Detection Range

12.35-1,000pg/mL

Sample Type

Tissue homogenates, Cell lysates, Cell culture supernates, Other biological fluids, Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Reagent Diluent	1×300µL	Stop Solution	1×6mL
TMB Substrate	1×9mL	Instruction manual	1
Wash Buffer (30 × concentrate)	1×20mL

Recovery

Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 2, Basic (FGF2) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 2, Basic (FGF2) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	87-95	91
EDTA plasma(n=5)	85-101	91
heparin plasma(n=5)	78-94	87

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 2, Basic (FGF2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	91-98%	96-105%	86-101%	82-97%
EDTA plasma(n=5)	85-95%	93-102%	95-102%	80-102%
heparin plasma(n=5)	80-93%	83-101%	82-90%	84-99%

Features

Detects small molecule

Species

Rat

Sensitivity

5.11pg/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 2, Basic (FGF2) and unlabeled Fibroblast Growth Factor 2, Basic (FGF2) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample.

Gene ID	54250
UniProt ID	P13109
Antigen	FGF2
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Applications	ELISA
Research Area	Cytokine
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb Ischemia Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate Neurotoxicity Effects of Hemostatic Agents on Fibroblast Cells

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SKU	Supplier No.	Size	Your price
BHE10102074	CEA551Ra	96T*1	$608	Add to Cart
BHE10102074	CEA551Ra	96T*5	$2736	Add to Cart
BHE10102074	CEA551Ra	96T*10	$5168	Add to Cart

ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2) (Rat)

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