Matrices listed below were spiked with certain level of IL-1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
96
EDTA plasma(n=5)
88-102
98
heparin plasma(n=5)
88-96
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-96%
85-104%
85-93%
EDTA plasma(n=5)
92-100%
85-101%
88-99%
heparin plasma(n=5)
90-99%
85-99%
87-98%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IL-1b ELISA Kit allows for the in vitro quantitative determination of IL-1b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Zebra fish IL-1b(Interleukin 1 Beta) ELISA Kit