Matrices listed below were spiked with certain level of VD and the recovery rates were calculated by comparing the measured value to the expected amount of VD in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
95
EDTA plasma(n=5)
91-102
97
heparin plasma(n=5)
89-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-105%
90-96%
88-104%
EDTA plasma(n=5)
85-97%
84-96%
82-94%
heparin plasma(n=5)
82-97%
80-97%
80-99%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
VD ELISA Kit allows for the in vitro quantitative determination of VD concentrations in serum, plasma, tissue homogenates and other biological fluids.