Matrices listed below were spiked with certain level of TBARS and the recovery rates were calculated by comparing the measured value to the expected amount of TBARS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-100
96
EDTA plasma(n=5)
85-93
89
heparin plasma(n=5)
87-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TBARS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-103%
88-104%
89-105%
EDTA plasma(n=5)
92-101%
88-100%
86-97%
heparin plasma(n=5)
87-95%
86-100%
86-100%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
TBARS ELISA Kit allows for the in vitro quantitative determination of TBARS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Reviews of TBARS (Thiobarbituric Acid Reactive Substance) ELISA Kit