Matrices listed below were spiked with certain level of T and the recovery rates were calculated by comparing the measured value to the expected amount of T in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
92
EDTA plasma(n=5)
87-98
93
heparin plasma(n=5)
90-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of T and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.