Matrices listed below were spiked with certain level of Spermine and the recovery rates were calculated by comparing the measured value to the expected amount of Spermine in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
92
EDTA plasma(n=5)
85-102
92
heparin plasma(n=5)
87-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Spermine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-97%
86-104%
94-103%
EDTA plasma(n=5)
83-97%
85-96%
85-100%
heparin plasma(n=5)
85-99%
81-98%
83-95%
Sensitivity
< 7.5ng/ml
Notes
For Research Use Only
Antigen
Spermine
Applications
ELISA
Applications Notes
Spermine ELISA Kit allows for the in vitro quantitative determination of Spermine concentrations in serum, plasma, tissue homogenates and other biological fluids.