SP (Substance P) ELISA Kit

Mfr.No.

E-EL-0067

Sensitivity

46.88 pg/mL

Detection Range

78.125~5000pg/mL, 78.13-5000 pg/mL

Reactivity

Universal

Assay Duration

2.5h

Assay Type

Competitive

Description

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SP. During the reaction, SP in the sample or standard competes with a fixed amount of SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color tus from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Detection Method

Colormetric

Kit Components

An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells x12 strips 48T: 8 wells x6 strips	-20°C, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100x)	96T: 1 vial, 120 uL 48T: 1 vial, 60 uL
Concentrated HRP Conjugate (100x)	96T: 1 vial, 120 uL 48T: 1 vial, 60 uL	-20°C(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25x)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8°C(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Recovery

80%-120%. The recovery of SP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-96	93
EDTA plasma(n=8)	99-109	104
Cell culture media(n=8)	90-100	96

Specificity

This kit recognizes SP in samples. No significant cross-reactivity or interference between SP and analogues was observed.

Precision

Both intra-CV and inter-CV are < 10%.
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	233.17	510.94	2017.45	206.77	515.17	2371.94
Standard deviation	12.87	23.61	104.91	12.18	23.85	125.24
CV (%)	5.52	4.62	5.20	5.89	4.63	5.28

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of SP concentrations in serum, plasma and other biological fluids.

UniProt ID	H:P20366, M:P41539, R:P06767

Applications	ELISA
Applications Notes	This ELISA kit applies to the in vitro quantitative determination of SP concentrations in serum, plasma and other biological fluids.
Research Area	Neuroscience, Cardiovascular

Storage	2-8°C/-20°C,12 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
BHE10307787	E-EL-0067	96T	$495		Add to Cart
BHE10307787	E-EL-0067	48T	$396		Add to Cart

SP (Substance P) ELISA Kit

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