Simian PAI1(Plasminogen Activator Inhibitor 1) ELISA Kit

Mfr.No.

ELK8093

Sensitivity

5.3 pg/mL

Detection Range

12.5-800 pg/mL

Reactivity

Simian

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor

Recovery

Matrices listed below were spiked with certain level of recombinant PAI1 and the recovery rates were calculated by comparing the measured value to the expected amount of PAI1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-90%	85%
EDTA plasma(n=5)	84-106%	100%
Heparin plasma(n=5)	87-93%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAI1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-97%	90-99%	82-101%	93-105%
EDTA plasma(n=5)	88-101%	85-93%	89-97%	85-94%
Heparin plasma(n=5)	87-96%	92-105%	89-102%	92-101%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
800.00	2.126	2.028
400.00	1.625	1.527
200.00	1.142	1.044
100.00	0.806	0.708
50.00	0.453	0.355
25.00	0.315	0.217
12.50	0.142	0.044
0.00	0.098	0.000

Limit Of Detection

800 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian PAI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian PAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian PAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian PAI1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Metabolic pathway;Hematology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208052	ELK8093	48T	$412		Add to Cart
BHE15208052	ELK8093	96T	$588		Add to Cart

Simian PAI1(Plasminogen Activator Inhibitor 1) ELISA Kit

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