Simian INS(Insulin) ELISA Kit

Mfr.No.

ELK8119

Sensitivity

4.49 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Simian

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

Insulin

Recovery

Matrices listed below were spiked with certain level of recombinant INS and the recovery rates were calculated by comparing the measured value to the expected amount of INS in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	88-101%	94%
Heparin plasma(n=5)	91-105%	98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of INS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	82-96%	85-96%	87-101%	89-97%
EDTA plasma(n=5)	86-97%	93-105%	82-93%	95-104%
Heparin plasma(n=5)	82-96%	85-98%	95-104%	82-96%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.954	1.873
500.00	1.497	1.416
250.00	1.135	1.054
125.00	0.775	0.694
62.50	0.487	0.406
31.25	0.379	0.298
15.63	0.156	0.075
0.00	0.081	0.000

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian INS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian INS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian INS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian INS in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Metabolic pathway;Endocrinology;Hormone metabolism;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208049	ELK8119	48T	$412		Add to Cart
BHE15208049	ELK8119	96T	$588		Add to Cart

Simian INS(Insulin) ELISA Kit

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