Simian alpha2M(Alpha-2-Macroglobulin) ELISA Kit

Mfr.No.

ELK8292

Sensitivity

3.5 ng/mL

Detection Range

7.82-500 ng/mL

Reactivity

Simian

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

alpha2MG; alpha2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Recovery

Matrices listed below were spiked with certain level of recombinant ?2M and the recovery rates were calculated by comparing the measured value to the expected amount of ?2M in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	80-95%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ?2M and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-97%	96-105%	91-103%	89-97%
EDTA plasma(n=5)	78-92%	79-93%	94-106%	83-97%
Heparin plasma(n=5)	80-92%	86-102%	87-103%	85-101%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
500.00	1.941	1.845
250.00	1.700	1.604
125.00	1.043	0.947
62.50	0.733	0.637
31.25	0.593	0.497
15.63	0.351	0.255
7.82	0.190	0.094
0.00	0.096	0.000

Sample Type

Cell lysates, Serum, Cell culture supeatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

500 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian alpha2M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian alpha2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian alpha2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian alpha2M in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Tumor immunity;Infection immunity;Hematology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208046	ELK8292	48T	$412		Add to Cart
BHE15208046	ELK8292	96T	$588		Add to Cart

Simian alpha2M(Alpha-2-Macroglobulin) ELISA Kit

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