Sheep Vasopressin-neurophysin 2-copeptin(AVP) ELISA kit

Sheep Vasopressin-neurophysin 2-copeptin(AVP) ELISA kit

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Mfr.No.

CSB-EL002466SH

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Sheep

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

arginine vasopressin, ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone, arginine vasopressin-neurophysin II, neurohypophyseal, vasopressin-neurophysin II-copeptin

Recovery

The recovery of Human fFN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-96
EDTA plasma (n=4)	89	82-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human fFN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	92
	Range %	84-96
1:4	Average %	94
	Range %	85-98
1:8	Average %	102
	Range %	98-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1600	2.498	2.295	2.397	2.233
800	1.860	1.966	1.913	1.749
400	1.318	1.415	1.367	1.203
200	0.843	0.857	0.850	0.686
100	0.555	0.536	0.546	0.382
50	0.356	0.378	0.367	0.203
25	0.262	0.274	0.268	0.104
0	0.164	0.163	0.164

Species

Ovis aries

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	AVP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508960	CSB-EL002466SH	96T	$790	Add to Cart
BHE10508960	CSB-EL002466SH	5x96T	$3792	Add to Cart
BHE10508960	CSB-EL002466SH	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.