Sheep Tumor Necrosis Factor alpha,TNF-alpha ELISA Kit

Mfr.No.

CSB-E13853Sh

Sensitivity

3.125 pg/mL

Detection Range

3.125 pg/mL-50 pg/mL

Reactivity

Sheep

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

tumor necrosis factor (TNF superfamily, member 2), DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha

Recovery

The recovery of rat AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	94-109
EDTA plasma (n=4)	97	87-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	106
	Range %	98-111
1:2	Average %	102
	Range %	95-107
1:4	Average %	97
	Range %	88-102
1:8	Average %	104
	Range %	98-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.198	2.105	2.152	2.042
750	1.844	1.803	1.824	1.714
375	1.426	1.376	1.401	1.291
187.5	1.120	1.087	1.104	0.994
94	0.650	0.641	0.646	0.536
47	0.470	0.456	0.463	0.353
23.5	0.315	0.307	0.311	0.201
0	0.108	0.112	0.110	?

Species

Ovis aries

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P23383

Applications	ELISA
Research Area	TNF

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508958	CSB-E13853Sh	96T	$835	Add to Cart
BHE10508958	CSB-E13853Sh	5x96T	$4008	Add to Cart
BHE10508958	CSB-E13853Sh	10x96T	$7932.50	Add to Cart

Sheep Tumor Necrosis Factor alpha,TNF-alpha ELISA Kit

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