Sheep thyroxine (T4) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027512SH

Sensitivity

20 ng/mL

Detection Range

20 ng/mL-320 ng/mL

Reactivity

Sheep

Assay Duration

1-5h

Alternative Names

thyroxine (T4)

Recovery

The recovery of human FGF9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	101
	Range %	95-105
1:4	Average %	94
	Range %	87-98
1:8	Average %	96
	Range %	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	T4

Storage	Store at 2-8°C. Please refer to protocol.

Reference	EFFECTS OF THE GENOTYPE AND THE SEASONS ON PHYSIOLOGICAL PARAMETERS RELATED WITH ADAPTABILITY IN SHEEP IN MEDITERRANEAN CLIMATE CONDITIONS Tufan Altin.et al,/,/

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