Sheep Serum Amyloid A,SAA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14973Sh

Alternative Names

serum amyloid A1, MGC111216, PIG4, SAA, TP53I4, tumor protein p53 inducible protein 4

Reactivity

Sheep

Detection Range

1.875 ng/mL-120 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human CLDN4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-97
EDTA plasma (n=4)	85	80-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-97
1:2	Average %	89
	Range %	84-95
1:4	Average %	97
	Range %	92-101
1:8	Average %	99
	Range %	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Sensitivity

0.47 ng/mL

Limit Of Detection

450 nm

UniProt ID	P42819
Antigen	serum amyloid A1

Applications

ELISA

Research Area

SAA1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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