Sheep Serotonin N-acetyltransferase

Mfr.No.

E0181Sh

Sensitivity

11.12ng/L

Detection Range

20-4500ng/L

Reactivity

Sheep

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

AANAT;AA-NAT;Aralkylamine N-acetyltransferase;Serotonin acetylase;Serotonin N-acetyltransferase

Molecular Weight

23077

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0181Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	882.81	49.19	5.57
Intra-Assay	2	18	1727.74	85.17	4.93
Intra-Assay	3	18	3201.54	136.96	4.28

Background

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

Protocol

These standard curves of E0181Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.138	2.103	2.073
	2.067
2400ng/L	1.334	1.415	1.385
	1.496
1200ng/L	0.551	0.654	0.624
	0.757
600ng/L	0.422	0.398	0.368
	0.374
300ng/L	0.145	0.172	0.142
	0.199
0ng/L	0.03	0.03	0
	0.029

Gene ID	443531
UniProt ID	Q29495

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113649	E0181Sh	48T	$320		Add to Cart
BHE12113649	E0181Sh	96T	$458		Add to Cart

Sheep Serotonin N-acetyltransferase - AANAT ELISA Kit

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