Matrices listed below were spiked with certain level of SELE and the recovery rates were calculated by comparing the measured value to the expected amount of SELE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
96-104
99
EDTA plasma(n=5)
87-96
90
heparin plasma(n=5)
87-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SELE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-97%
85-101%
85-101%
EDTA plasma(n=5)
84-99%
84-98%
86-99%
heparin plasma(n=5)
83-99%
86-100%
88-98%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q95LG2
Applications
ELISA
Applications Notes
SELE ELISA Kit allows for the in vitro quantitative determination of SELE concentrations in serum, plasma, tissue homogenates and other biological fluids.