Matrices listed below were spiked with certain level of S100B and the recovery rates were calculated by comparing the measured value to the expected amount of S100B in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-99
93
EDTA plasma(n=5)
85-100
95
heparin plasma(n=5)
93-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-101%
89-102%
86-104%
EDTA plasma(n=5)
82-88%
83-97%
86-101%
heparin plasma(n=5)
85-97%
81-93%
83-96%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
S100B ELISA Kit allows for the in vitro quantitative determination of S100B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Sheep S100B(S100 Calcium Binding Protein B) ELISA Kit