Sheep Progesterone(PROG) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13176Sh

Sensitivity

0.2 ng/mL

Detection Range

0.4 ng/mL-30 ng/mL

Reactivity

Sheep

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Progesterone(PROG)

Recovery

The recovery of human SFMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-91
EDTA plasma (n=4)	99	95-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SFMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	100
	Range %	95-106
1:4	Average %	96
	Range %	92-100
1:8	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PROG

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dose-dependent effects of luteinizing hormone and follicle stimulating hormone on in vitro maturation, apoptosis, secretion function and expression of follicle Wei, S.et al,South African Journal of Animal Science,/

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