Sheep Progesterone(PROG) ELISA Kit

Sheep Progesterone(PROG) ELISA Kit

Reviews of Sheep Progesterone(PROG) ELISA Kit

Rate it now..!!

Rate it now..!!

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Mfr.No.

CSB-E13176Sh

Alternative Names

Progesterone(PROG)

Reactivity

Sheep

Detection Range

0.4 ng/mL-30 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human SFMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-91
EDTA plasma (n=4)	99	95-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SFMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	100
	Range %	95-106
1:4	Average %	96
	Range %	92-100
1:8	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average	Corrected
400	1.992	1.952	1.972	1.839
200	1.426	1.436	1.431	1.298
100	1.007	1.093	1.050	0.917
50	0.618	0.625	0.622	0.489
25	0.351	0.361	0.356	0.223
12.5	0.263	0.274	0.269	0.136
6.25	0.205	0.215	0.210	0.077
0	0.132	0.134	0.133	?

Species

Ovis aries

Sensitivity

0.2 ng/mL

Limit Of Detection

450 nm

Antigen	Progesterone(PROG)

Applications

ELISA

Research Area

PROG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dose-dependent effects of luteinizing hormone and follicle stimulating hormone on in vitro maturation, apoptosis, secretion function and expression of follicle Wei, S.et al,South African Journal of Animal Science,/

No Description Available.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10508942	CSB-E13176Sh	96T	$495	Add to Cart
BHE10508942	CSB-E13176Sh	5x96T	$2376	Add to Cart
BHE10508942	CSB-E13176Sh	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.