Matrices listed below were spiked with certain level of NOS3 and the recovery rates were calculated by comparing the measured value to the expected amount of NOS3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
95
EDTA plasma(n=5)
90-103
95
heparin plasma(n=5)
94-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-98%
89-99%
90-105%
EDTA plasma(n=5)
82-98%
82-96%
82-94%
heparin plasma(n=5)
82-97%
82-96%
81-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P79209
Applications
ELISA
Applications Notes
NOS3 ELISA Kit allows for the in vitro quantitative determination of NOS3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Sheep NOS3(Nitric oxide synthase, endothelial) ELISA Kit