Sheep Interleukin 8 - IL-8 ELISA Kit

Mfr.No.

E0073Sh

Description

This sandwich kit is for the accurate quantitative detection of Interleukin 8 (also known as IL-8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Chemokine;C-X-C motif chemokine 8;C-X-C motif ligand 8;IL 8;IL8;IL-8;Interleukin-8

Reactivity

Sheep

Detection Range

3-900ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

11292

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.62ng/L

Background

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Gene ID	443418
UniProt ID	P36925
Antigen	IL-8

Research Area

Cardiovascular; Immunology

Protocol

These standard curves of E0073Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.206	2.307	2.282
	2.408
240ng/L	1.192	1.195	1.17
	1.198
120ng/L	0.748	0.765	0.741
	0.783
60ng/L	0.443	0.416	0.391
	0.389
30ng/L	0.19	0.182	0.157
	0.173
0ng/L	0.019	0.025	0
	0.031

Precision

We measured random samples of E0073Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	84.17	4.72	5.61
Intra-Assay	2	18	354.46	6.89	1.94
Intra-Assay	3	18	167.36	8.44	5.04

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113547	E0073Sh	48T	$320		Add to Cart
BHE12113547	E0073Sh	96T	$458		Add to Cart

Sheep Interleukin 8 - IL-8 ELISA Kit

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