Sheep Interleukin-8,IL-8 ELISA Kit

Sheep Interleukin-8,IL-8 ELISA Kit

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Mfr.No.

CSB-E13202Sh

Alternative Names

interleukin 8, CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, T cell chemotactic factor, beta-thromboglobulin-like protein, chemokine (C-X-C motif) ligand 8, emoctakin, granulocyte chemotactic

Reactivity

Sheep

Detection Range

6.25 pg/mL-400 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of chicken PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	93	90-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-99
1:2	Average %	97
	Range %	92-103
1:4	Average %	86
	Range %	80-89
1:8	Average %	90
	Range %	83-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
800	0.220	0.213	0.217
400	0.359	0.334	0.347
200	0.607	0.621	0.614
100	1.118	1.120	1.119
50	2.228	2.189	2.209

Species

Ovis aries

Sensitivity

1.56 pg/mL

Limit Of Detection

450 nm

UniProt ID	P36925
Antigen	Interleukin 8

Applications

ELISA

Research Area

IL8

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508918	CSB-E13202Sh	96T	$658	Add to Cart
BHE10508918	CSB-E13202Sh	5x96T	$3158.40	Add to Cart
BHE10508918	CSB-E13202Sh	10x96T	$6251	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.