Sheep Interleukin 4(IL-4)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15963Sh

Alternative Names

interleukin 4, BCGF-1, BCGF1, BSF1, IL-4, MGC79402, B cell growth factor 1, B_cell stimulatory factor 1, lymphocyte stimulatory factor 1

Reactivity

Sheep

Detection Range

6.25 pg/mL-400 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rabbit LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-104
EDTA plasma (n=4)	100	94-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	90
	Range %	84-95
1:4	Average %	96
	Range %	89-102
1:8	Average %	98
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Sensitivity

1.56 pg/mL

Limit Of Detection

450 nm

UniProt ID	P30368
Antigen	Interleukin 4

Applications

ELISA

Research Area

IL4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508914	CSB-E15963Sh	96T	$695	Add to Cart
BHE10508914	CSB-E15963Sh	5x96T	$3336	Add to Cart
BHE10508914	CSB-E15963Sh	10x96T	$6602.50	Add to Cart