Sheep Interleukin 10,IL-10 ELISA Kit

Mfr.No.

CSB-E12817Sh

Alternative Names

interleukin 10, CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor

Reactivity

Sheep

Detection Range

5 pg/mL-80 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rat ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-94
EDTA plasma (n=4)	103	92-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	104
	Range %	93-109
1:2	Average %	112
	Range %	103-116
1:4	Average %	94
	Range %	84-98
1:8	Average %	90
	Range %	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Sensitivity

5 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q29408
Antigen	Interleukin 10

Applications

ELISA

Research Area

IL10

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508902	CSB-E12817Sh	96T	$835	Add to Cart
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BHE10508902	CSB-E12817Sh	10x96T	$7932.50	Add to Cart