Sheep Insulin-like growth factor II(IGF2) ELISA kit

Sheep Insulin-like growth factor II(IGF2) ELISA kit

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Mfr.No.

CSB-EL011088SH

Sensitivity

3.9 ng/mL

Detection Range

15.6 ng/mL-1000 ng/mL

Reactivity

Sheep

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

insulin-like growth factor 2 (somatomedin A), C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, OTTHUMP00000011018, OTTHUMP00000011157, insulin-like growth factor 2, insulin-like growth factor II, insulin-like growth factor type 2, putative insulin-like

Recovery

The recovery of rat TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-92
EDTA plasma (n=4)	99	95-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	99
	Range %	95-104
1:4	Average %	101
	Range %	97-105
1:8	Average %	98
	Range %	93-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.037	2.067	2.052	1.871
200	1.445	1.489	1.467	1.286
100	1.017	1.034	1.026	0.845
50	0.655	0.677	0.666	0.485
25	0.411	0.438	0.425	0.244
12.5	0.351	0.378	0.365	0.184
6.25	0.297	0.282	0.290	0.109
0	0.183	0.179	0.181	?

Species

Ovis aries

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P10764

Applications	ELISA
Research Area	IGF2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508900	CSB-EL011088SH	96T	$595	Add to Cart
BHE10508900	CSB-EL011088SH	5x96T	$2856	Add to Cart
BHE10508900	CSB-EL011088SH	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.