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Home / ELISA Kit

Sheep Immunoglobulin M(IgM) ELISA Kit

SKU : BHE10508895

This Human DDIT4 ELISA Kit was designed for the quantitative measurement of Human DDIT4 protein in serum, tissue homogenates, cell lysates, urine, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E13682Sh

Sensitivity

1.25 ug/mL

Detection Range

2.5 ug/mL-40 ug/mL

Reactivity

Sheep

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Immunoglobulin M(IgM)

Recovery

The recovery of human DDIT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DDIT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		89
		Range %		86-92
1:2		Average %		94
		Range %		89-98
1:4		Average %		96
		Range %		92-99
1:8		Average %		98
		Range %		92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.948	2.754	2.851	2.691
5	2.539	2.412	2.476	2.316
2.5	1.781	1.662	1.722	1.562
1.25	1.248	1.198	1.223	1.063
0.625	0.738	0.706	0.722	0.562
0.312	0.514	0.504	0.509	0.349
0.156	0.305	0.298	0.302	0.142
0	0.162	0.158	0.160	?

Species

Ovis aries

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

Applications	ELISA
Research Area	IgM

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Colostral immunoglobulin concentration and milk production of ewes fed salt tolerant forages as alternatives to berseem hay A.S.Morsy.et al,Livestock Science ,Feb 22

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SKU	Supplier No.	Size	Your price	Quantity
BHE10508895	CSB-E13682Sh	96T	$790		Add to Cart
BHE10508895	CSB-E13682Sh	5x96T	$3792		Add to Cart
BHE10508895	CSB-E13682Sh	10x96T	$7505		Add to Cart

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