Sheep Immunoglobulin A(IgA) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13681Sh

Sensitivity

1.87 ug/mL

Detection Range

3.75 ug/mL-60 ug/mL

Reactivity

Sheep

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Immunoglobulin A(IgA)

Recovery

The recovery of human DBH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	97	90-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DBH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-96
1:2	Average %	95
	Range %	91-100
1:4	Average %	98
	Range %	91-105
1:8	Average %	93
	Range %	84-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Immune response of sheep naturally infected with Haemonchus spp. on pastures with two different nutritional conditions Gustavo Toscan.et al,SEMIN-CIENC AGRAR,May 5 Effects of herbal nutraceuticals and/or zinc against Haemonchus contortus in lambs experimentally infected Vradyov Z.et al,BMC Vet Res,Mar 9

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BHE10508892	CSB-E13681Sh	10x96T	$7505	Add to Cart