Sheep Immunoglobulin A(IgA) ELISA Kit

Sheep Immunoglobulin A(IgA) ELISA Kit

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Mfr.No.

CSB-E13681Sh

Alternative Names

Immunoglobulin A(IgA)

Reactivity

Sheep

Detection Range

3.75 ug/mL-60 ug/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human DBH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	97	90-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DBH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-96
1:2	Average %	95
	Range %	91-100
1:4	Average %	98
	Range %	91-105
1:8	Average %	93
	Range %	84-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
0	1.958	1.963	1.961
2.5	1.311	1.321	1.316
10	0.812	0.836	0.824
40	0.516	0.527	0.522
125	0.319	0.324	0.322
500	0.186	0.189	0.188

Species

Ovis aries

Sensitivity

1.87 ug/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin A(IgA)

Applications

ELISA

Research Area

IgA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Immune response of sheep naturally infected with Haemonchus spp. on pastures with two different nutritional conditions Gustavo Toscan.et al,SEMIN-CIENC AGRAR,May 5 Effects of herbal nutraceuticals and/or zinc against Haemonchus contortus in lambs experimentally infected Vradyov Z.et al,BMC Vet Res,Mar 9

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SKU	Supplier No.	Size	Your price
BHE10508892	CSB-E13681Sh	96T	$790	Add to Cart
BHE10508892	CSB-E13681Sh	5x96T	$3792	Add to Cart
BHE10508892	CSB-E13681Sh	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.