Matrices listed below were spiked with certain level of IL-5 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
92
EDTA plasma(n=5)
93-104
100
heparin plasma(n=5)
85-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-5 ELISA Kit allows for the in vitro quantitative determination of IL-5 concentrations in serum, plasma, tissue homogenates and other biological fluids.