Matrices listed below were spiked with certain level of IgG1 and the recovery rates were calculated by comparing the measured value to the expected amount of IgG1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
93
EDTA plasma(n=5)
95-102
98
heparin plasma(n=5)
88-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IgG1 ELISA Kit allows for the in vitro quantitative determination of IgG1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Sheep IgG1(Immunoglobulin G1) ELISA Kit