Matrices listed below were spiked with certain level of HLA-DQB2 and the recovery rates were calculated by comparing the measured value to the expected amount of HLA-DQB2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
95
EDTA plasma(n=5)
91-99
96
heparin plasma(n=5)
88-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HLA-DQB2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-105%
89-102%
85-99%
EDTA plasma(n=5)
86-97%
88-96%
85-101%
heparin plasma(n=5)
89-100%
88-96%
85-99%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
HLA-DQB2 ELISA Kit allows for the in vitro quantitative determination of HLA-DQB2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Sheep HLA-DQB2(HLA class II histocompatibility antigen, DQ beta 2 chain) ELISA Kit