Sheep Erythropoietin - EPO ELISA Kit

Mfr.No.

E0087Sh

Sensitivity

0.048ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Sheep

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Erythropoietin (also known as EPO) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

EPO;Erythropoietin;MVCD2

Molecular Weight

21335

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0087Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.32	0.67	4.7
Intra-Assay	2	18	23.35	1.08	4.62
Intra-Assay	3	18	7.81	0.38	4.89

Background

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Protocol

These standard curves of E0087Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	1.689	1.742	1.711
	1.795
12ng/mL	1.005	1.02	0.989
	1.034
6ng/mL	0.632	0.585	0.554
	0.537
3ng/mL	0.267	0.203	0.172
	0.139
1.5ng/mL	0.073	0.107	0.076
	0.141
0ng/mL	0.032	0.031	0
	0.03

Gene ID	443302
UniProt ID	P33709

Research Area	Cardiovascular; Signal Transduction; Stem Cells; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113561	E0087Sh	48T	$320		Add to Cart
BHE12113561	E0087Sh	96T	$458		Add to Cart

Sheep Erythropoietin - EPO ELISA Kit

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