Matrices listed below were spiked with certain level of Dyn and the recovery rates were calculated by comparing the measured value to the expected amount of Dyn in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
95
EDTA plasma(n=5)
90-105
98
heparin plasma(n=5)
90-98
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dyn and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-101%
85-105%
86-101%
EDTA plasma(n=5)
82-98%
85-100%
82-100%
heparin plasma(n=5)
87-100%
80-100%
84-100%
Sensitivity
< 4.688pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Dyn ELISA Kit allows for the in vitro quantitative determination of Dyn concentrations in serum, plasma, tissue homogenates and other biological fluids.