Matrices listed below were spiked with certain level of AMH and the recovery rates were calculated by comparing the measured value to the expected amount of AMH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
94
EDTA plasma(n=5)
85-100
94
heparin plasma(n=5)
85-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
96-105%
85-101%
91-101%
EDTA plasma(n=5)
85-100%
85-99%
86-98%
heparin plasma(n=5)
86-100%
89-100%
86-98%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
AMH ELISA Kit allows for the in vitro quantitative determination of AMH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Sheep AMH(Muellerian-inhibiting factor) ELISA Kit