Sheep adrenocorticotropic hormone (ACTH) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027618SH

Sensitivity

0.097 pg/mL

Detection Range

0.097 pg/mL-400 pg/mL

Reactivity

Sheep

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

adrencocorticotropic hormone (ACTH)

Recovery

The recovery of mouse C5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	87-101
EDTA plasma (n=4)	98	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	86-100
1:200	Average %	95
	Range %	87-106
1:400	Average %	90
	Range %	86-99
1:800	Average %	95
	Range %	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	ACTH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of Salsolinol on ACTH and Cortisol Response to Handling Stress in Early Anestrous Sheep Magorzata Hasiec.et al,Czech J. Anim. Sci.,2017

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